Sapio, Daniela (2013) Effects of bone mesenchymal stem cells (BMSCs) on the rat pial microvascular remodeling after transient middle cerebral artery occlusion and different reperfusion times. [Tesi di dottorato]

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Item Type: Tesi di dottorato
Lingua: English
Title: Effects of bone mesenchymal stem cells (BMSCs) on the rat pial microvascular remodeling after transient middle cerebral artery occlusion and different reperfusion times
Creators:
CreatorsEmail
Sapio, Danieladaniela.sapio@unina.it
Date: 2 April 2013
Number of Pages: 54
Institution: Università degli Studi di Napoli Federico II
Department: Medicina Clinica e Chirurgia
Scuola di dottorato: Medicina molecolare
Dottorato: Neuroscienze
Ciclo di dottorato: 25
Coordinatore del Corso di dottorato:
nomeemail
Annunziato, Luciofarmacol@unina.it
Tutor:
nomeemail
Colantuoni, Antoniocolantuo@unina.it
Date: 2 April 2013
Number of Pages: 54
Uncontrolled Keywords: Pial circulation; MCAO; bone mesenchymal stem cells
Settori scientifico-disciplinari del MIUR: Area 05 - Scienze biologiche > BIO/09 - Fisiologia
Aree tematiche (7° programma Quadro): SALUTE e TUTELA DEL CONSUMATORE > Biotecnologie, strumenti e tecnologie generiche per la salute umana
Date Deposited: 10 Apr 2013 09:19
Last Modified: 17 Jun 2014 06:04
URI: http://www.fedoa.unina.it/id/eprint/9233

Abstract

Transplantation of bone mesenchymal stem cells (BMSCs) represent a potential therapy for ischemic stroke, we sought to evaluate the long-term effects of these cells treatment on rat pial microcirculation after middle cerebral artery occlusion (MCAO). Male rats were subjected to 2-hours MCAO followed by an infusion of 1 x 106 BMSCs or phosphate-buffered saline into internal carotid artery. Pial microcirculation was observed through a closed cranial window at different reperfusion time (7, 14 and 28 days) by fluorescence microscopy. Geometric characteristics of pial arteriolar networks, permeability increase, leukocyte adhesion, perfused capillary density were analyzed. The expression of the most important proteins in the process of angiogenesis was evaluated by western blot analysis. Finally, we performed green fluorescent protein (GFP)-BMSCs to transplanted to follow their distribution on infarcted area. The post-ischemic penumbra induced by MCAO is a site of intense microvascular reorganization, characterized by new vessel connections resulting in several anastomotic arterioles. The intracarotid administration of bone mesenchymal stem cells up to 28 days post middle cerebral artery occlusion induced an accelerated pial vascular remodeling. These arcades were likely vessels sprouting from preexistent arterioles localized in the penumbra area, able to place on the ischemic core, accompanied by increased expression of vascular endothelial growth factor and endothelial nitric oxide synthase. GFP-BMSCs showed the distribution and organization in infarcted area up to 28 days of reperfusion. Pial vascular remodeling after MCAO mainly involves arteriolar networks; the graft of bone mesenchymal stem cells accelerates this processes through angiogenic mechanisms.

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