Stefanetti, Giuseppe (2014) THE IMPACT OF SALMONELLA POLYSACCHARIDE ANTIGENS STRUCTURE AND CONJUGATION CHEMISTRY ON NTS GLYCOCONJUGATE VACCINES. [Tesi di dottorato]
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Giuseppe Stefanetti - PhD Thesis.pdf Download (4MB) | Anteprima |
| Tipologia del documento: | Tesi di dottorato |
|---|---|
| Lingua: | English |
| Titolo: | THE IMPACT OF SALMONELLA POLYSACCHARIDE ANTIGENS STRUCTURE AND CONJUGATION CHEMISTRY ON NTS GLYCOCONJUGATE VACCINES |
| Autori: | Autore Email Stefanetti, Giuseppe stefanetti.g@gmail.com |
| Data: | 26 Marzo 2014 |
| Numero di pagine: | 113 |
| Istituzione: | Università degli Studi di Napoli Federico II |
| Dipartimento: | Scienze Chimiche |
| Scuola di dottorato: | Biotecnologie |
| Dottorato: | Scienze biotecnologiche |
| Ciclo di dottorato: | 26 |
| Coordinatore del Corso di dottorato: | nome email Sannia, Giovanni sannia@unina.it |
| Tutor: | nome email Sannia, Giovanni [non definito] Micoli, Francesca [non definito] |
| Data: | 26 Marzo 2014 |
| Numero di pagine: | 113 |
| Parole chiave: | Vaccines, Glycoconjugates, Nontyphoidal Salmonella, Carbohydrates |
| Settori scientifico-disciplinari del MIUR: | Area 05 - Scienze biologiche > BIO/11 - Biologia molecolare |
| Aree tematiche (7° programma Quadro): | SALUTE e TUTELA DEL CONSUMATORE > Biotecnologie, strumenti e tecnologie generiche per la salute umana |
| Depositato il: | 08 Apr 2014 11:05 |
| Ultima modifica: | 15 Lug 2015 01:01 |
| URI: | http://www.fedoa.unina.it/id/eprint/9658 |
Abstract
The Novartis Vaccines Institute for Global Health (NVGH) is working on the development of a bivalent glycoconjugate vaccine against nontyphoidal Salmonellae (NTS) covering the main serovars S. Typhimurium and S. Enteritidis causing invasive disease in Africa. The vaccine is based on the conjugation of the lipopolysaccharide O antigen, the main surface polysaccharide of NTS, with CRM197 as carrier protein. In this contest, my PhD project has been focused in three related aspects: 1) Structural and biological characterization of NTS O antigen purified from different S. Typhimurium and S. Enteritidis strains. 2) Synthesis, characterization and immunological evaluation of a panel of NTS glycoconjugate vaccine variants (OAg source, conjugation chemistry, OAg/protein ratio) to better understand the key factors in glycoconjugate vaccine design required for optimal antibody-mediated immunity. 3) Design of new efficient glycoconjugation methodologies which allow the synthesis of structurally highly-defined glycoconjugate vaccines and simplify the conjugation process.
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