Esposito, Davide (2014) Human rpL3 regulates DNA damage reponse by modulating p21(waf1/cip1) levels in a p53-independent manner. [Tesi di dottorato]
Preview |
Text
Davide_Esposito_26.pdf Download (9MB) | Preview |
| Item Type: | Tesi di dottorato |
|---|---|
| Resource language: | English |
| Title: | Human rpL3 regulates DNA damage reponse by modulating p21(waf1/cip1) levels in a p53-independent manner |
| Creators: | Creators Email Esposito, Davide davide.esposito@mssn.edu |
| Date: | 30 March 2014 |
| Number of Pages: | 66 |
| Institution: | Università degli Studi di Napoli Federico II |
| Department: | Medicina Molecolare e Biotecnologie Mediche |
| Scuola di dottorato: | Scienze biologiche |
| Dottorato: | Biochimica e biologia cellulare e molecolare |
| Ciclo di dottorato: | 26 |
| Coordinatore del Corso di dottorato: | nome email Arcari, Paolo paolo.arcari@unina.it |
| Tutor: | nome email Russo, Giulia UNSPECIFIED Missero, Caterina UNSPECIFIED |
| Date: | 30 March 2014 |
| Number of Pages: | 66 |
| Keywords: | rpL3, extraribosomal function, ribosomal stress, DNA damage, p21 |
| Settori scientifico-disciplinari del MIUR: | Area 05 - Scienze biologiche > BIO/10 - Biochimica Area 05 - Scienze biologiche > BIO/11 - Biologia molecolare |
| Aree tematiche (7° programma Quadro): | SALUTE e TUTELA DEL CONSUMATORE > Biotecnologie, strumenti e tecnologie generiche per la salute umana |
| Date Deposited: | 09 Apr 2014 10:17 |
| Last Modified: | 26 Jan 2015 11:45 |
| URI: | http://www.fedoa.unina.it/id/eprint/9818 |
Collection description
It is now largely accepted that ribosomal proteins may be implicated in a variety of biological functions besides that of components of the translation machinery. Many evidences show that a subset of ribosomal proteins are involved in the regulation of the cell cycle and apoptosis through modulation of p53 activity. In addition, p53-independent mechanisms of cell cycle arrest in response to alterations of ribosomal proteins availability have been described. Here, I identify human rpL3 as a new regulator of cell cycle and apoptosis through the regulation of p21 expression in a p53-independent system. In particular, DNA damage induces accumulation of free-ribosome rpL3. This amount of rpL3, regulating p21 expression, is responsible for cell cycle arrest in response to a mild DNA damage, and apoptosis when the damage in higher and not reparable. In addition, the role of rpL3 is partially independent of p21. Taken together, these findings unravel a novel extraribosomal function of rpL3 which acts as ribosomal stress mediator inhibiting cell proliferation or triggering apoptosis in dependence of the nature of the stress.
Downloads
Downloads per month over past year
Actions (login required)
 |
View Item |
