Alterazioni strutturali della lamina basale in lesioni aneurismatiche asimmetriche dell'aorta ascendente

Guerra, Germano (2006) Alterazioni strutturali della lamina basale in lesioni aneurismatiche asimmetriche dell'aorta ascendente. [Tesi di dottorato] (Inedito)

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Abstract

Basal lamina (BL) is a crucial mechanical and functional component of blood vessels, constituting a sensor of extracellular microenvironment for endothelial cells and pericytes. Recently, an abnormality in the process of matrix microfibrillar component remodeling has been advocated as a mechanism involved in the development of aortic dilation. We focused our attention on BL composition and organization and studied some of the main components of the Extracellular Matrix such as Tenascin, Laminins, Fibronectin, type I, III and IV Collagens. We used surgical fragments from 27 patients, submitted to operation because of aortic root aneurysm and 5 normal aortic wall specimens from heart donors without any evidence for aneurysmal or atherosclerotic diseases of the aorta. Two samples of aortic wall were harvested from each patient, proximal to the sinotubular junction at the aortic convexity and concavity. Each specimen was processed both for immunohistochemical examination and molecular biology study. We compared the convexity of each aortic sample with the concavity of the same vessel, and both of them with the control samples. The synthesis of mRNA and the levels of each protein were assessed, respectively, by RT-PCR and Western Blot analysis. Immunohistochemistry elucidated the organization of BL, whose composition was revealed by molecular biology. All pathological samples showed a wall thinner than normal ones. Basal lamina of the aortic wall evidentiated important changes in the tridimensional arrangement of its major components which lost their regular arrangement in pathological specimens. Collagen I, Laminin 2 chain and Fibronectin amounts decreased in pathological samples, while type IV Collagen and Tenascin synthesis increased. Consistently with the common macroscopic observation that ascending aorta dilations tend to expand asymmetrically, with prevalent involvement of the vessel convexity and relative sparing of the concavity, Collagen type IV is more evident in the concavity and Tenascin in the convexity.

Tipologia di documento:Tesi di dottorato
Parole chiave:ExtraCellular matrix, Aortic dilation, Basal lamina
Settori scientifico-disciplinari MIUR:Area 05 Scienze biologiche > BIO/17 ISTOLOGIA
Area 06 Scienze mediche > MED/05 PATOLOGIA CLINICA
Area 06 Scienze mediche > MED/03 GENETICA MEDICA
Area 06 Scienze mediche > MED/36 DIAGNOSTICA PER IMMAGINI E RADIOTERAPIA
Area 06 Scienze mediche > MED/08 ANATOMIA PATOLOGICA
Area 06 Scienze mediche > MED/46 SCIENZE TECNICHE DI MEDICINA DI LABORATORIO
Area 05 Scienze biologiche > BIO/16 ANATOMIA UMANA
Coordinatori della Scuola di dottorato:
Coordinatore del Corso di dottoratoe-mail (se nota)
Montagnani, Stefania
Tutor della Scuola di dottorato:
Tutor del Corso di dottoratoe-mail (se nota)
Montagnani, Stefania
Stato del full text:Accessibile
Data:2006
Numero di pagine:45
Istituzione:Università degli Studi di Napoli Federico II
Dipartimento o Struttura:Scienze biomorfologiche e funzionali
Tipo di tesi:Dottorato
Stato dell'Eprint:Inedito
Denominazione del dottorato:Morfologia clinica e patologica
Ciclo di dottorato:XVII
Numero di sistema:990
Depositato il:01 Agosto 2008
Ultima modifica:04 Febbraio 2009 09:41

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