Carraturo, Federica (2017) Metabolomic profiling and molecular characterization of food matrices: identification of potential markers of microbial contamination. [Tesi di dottorato]
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PhD_FedericaCarraturo.pdf Download (5MB) | Anteprima |
| Tipologia del documento: | Tesi di dottorato | ||||
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| Lingua: | English | ||||
| Titolo: | Metabolomic profiling and molecular characterization of food matrices: identification of potential markers of microbial contamination | ||||
| Autori: |
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| Data: | 31 Marzo 2017 | ||||
| Numero di pagine: | 99 | ||||
| Istituzione: | Università degli Studi di Napoli Federico II | ||||
| Dipartimento: | Sanità Pubblica | ||||
| Scuola di dottorato: | Medicina preventiva, pubblica e sociale | ||||
| Dottorato: | Sanità pubblica e medicina preventiva | ||||
| Ciclo di dottorato: | 29 | ||||
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| Data: | 31 Marzo 2017 | ||||
| Numero di pagine: | 99 | ||||
| Parole chiave: | metabolomics, food, microbiology, GC-MS, Salmonella, Campylobacter, S.aureus | ||||
| Settori scientifico-disciplinari del MIUR: | Area 05 - Scienze biologiche > BIO/19 - Microbiologia generale Area 03 - Scienze chimiche > CHIM/10 - Chimica degli alimenti Area 06 - Scienze mediche > MED/07 - Microbiologia e microbiologia clinica Area 06 - Scienze mediche > MED/42 - Igiene generale e applicat |
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| Depositato il: | 20 Apr 2017 10:24 | ||||
| Ultima modifica: | 13 Mar 2018 11:49 | ||||
| URI: | http://www.fedoa.unina.it/id/eprint/11474 | ||||
| DOI: | 10.6093/UNINA/FEDOA/11474 |
Abstract
The research aims to generate an early warning system able to highlight, in real time, bacterial contamination of meat matrices and to provide information which could support companies in accepting or rejecting batches. Current microorganisms detection methods rely on techniques (plate counting), which provide retrospective values for microbial contamination. The disposal of fast headspace air measurement, using gas chromatography-mass spectrometry analysis, able to accurately and rapidly (30 min per sample) detect microbial spoilage in raw meat, could result a valid replacement to traditional and time-consuming (3 to 4 days) standardized microbiological analysis required by regulations. The experiments focused on the qualitative and quantitative evaluation of volatile organic compounds (VOCs) produced by Salmonella Typhimurium, Campylobacter jejuni and Staphylococcus aureus in different types of raw meat (beef, pork, chicken). The reduction of analysis times represents the strength of the alternative method to ISO protocols, although these are currently the official procedures provided by International regulations. The applied method requires smaller sample aliquots and does not need any sample processing, thus consenting its application on different food matrices (not only meat) and for the detection of a wide variety of pathogens. The validation of the suggested analytical approach would therefore result innovative, by evaluating further samples in order to demonstrate the benefits of the technique in terms of times, costs and preservation of consumers health. Data analysis allowed the characterization of unique VOC profiles and possible marker compounds of meat contamination due to certain pathogens. The identification of marker volatile compounds resulted essential to outline specific metabolic profiles for each microorganism responsible of meat spoilage.
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