Di Stasio, Luigia (2018) Intolerances and food allergies: assessment of the stability of allergenic proteins to gastrointestinal digestion. [Tesi di dottorato]
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| Tipologia del documento: | Tesi di dottorato |
|---|---|
| Lingua: | English |
| Titolo: | Intolerances and food allergies: assessment of the stability of allergenic proteins to gastrointestinal digestion |
| Autori: | Autore Email Di Stasio, Luigia luigia.distasio@unina.it |
| Data: | 10 Dicembre 2018 |
| Numero di pagine: | 149 |
| Istituzione: | Università degli Studi di Napoli Federico II |
| Dipartimento: | Agraria |
| Dottorato: | Scienze agrarie e agroalimentari |
| Ciclo di dottorato: | 31 |
| Coordinatore del Corso di dottorato: | nome email D'Urso, Guido durso@unina.it |
| Tutor: | nome email Ferranti, Pasquale [non definito] |
| Data: | 10 Dicembre 2018 |
| Numero di pagine: | 149 |
| Parole chiave: | food allergy, food intolerance, digestion |
| Settori scientifico-disciplinari del MIUR: | Area 07 - Scienze agrarie e veterinarie > AGR/15 - Scienze e tecnologie alimentari |
| Depositato il: | 03 Gen 2019 17:13 |
| Ultima modifica: | 23 Giu 2020 10:00 |
| URI: | http://www.fedoa.unina.it/id/eprint/12564 |
Abstract
The objectives of the present PhD project are summarized as follow: A1) evaluation of the digestion stability of major nut allergens (peanuts, hazelnuts, walnuts and almonds) as whole food, using an in vitro static model that simulates the gastrointestinal digestion process, including oral, gastric, duodenal and intestinal (brush border membrane enzymes) phases; A2) determine the stability of nut allergens following gastrointestinal digestion of whole food using proteomic techniques (SDS-PAGE, RP-HPLC, LC- HR-MS/MS); A3) assess the allergenic properties of nuts following gastrointestinal digestion of whole food using immunological methodology (ELISA, western-blot, dot-blot, RBL assay); A4) investigate how in vitro gastro-intestinal digestion affects the immune toxic properties of gliadin from diploid (Triticum monococcum) compared to hexaploid (Triticum aestivum) wheat by an immunological and proteomic approach.
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