Conduto Dias Maia, Ana Raquel (2020) Development of a new vaccination strategy against Clostridium difficile infection. [Tesi di dottorato]


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Item Type: Tesi di dottorato
Resource language: English
Title: Development of a new vaccination strategy against Clostridium difficile infection
Conduto Dias Maia, Ana
Date: March 2020
Number of Pages: 101
Institution: Università degli Studi di Napoli Federico II
Department: Biologia
Dottorato: Biologia
Ciclo di dottorato: 32
Coordinatore del Corso di dottorato:
Date: March 2020
Number of Pages: 101
Keywords: Clostidium difficile infection, mucosal vaccine, Bacillus subtilis, Bacillus collagen-like protein, Exosporium Cysteine rich protein
Settori scientifico-disciplinari del MIUR: Area 05 - Scienze biologiche > BIO/19 - Microbiologia generale
Date Deposited: 26 Mar 2020 08:34
Last Modified: 08 Nov 2021 12:11

Collection description

Despite the emergence of new anti-Clostridium difficile infection (CDI) therapies and improvement of health care systems around the world, the incidence of CDI has increased over 200% since 2000 and it is now one of the leading causes of healthcare-associated infections. The main aim of this project was to develop a mucosal vaccine against C. difficile spores, the vehicle of transmission, infection and perpetuation of the disease. The C-terminal domain of the exosporium proteins BclA2 and BclA3 form C. difficile spores were chosen as potential antigens for the nasal immunization of mice. Both antigens where used as free proteins and bound to B. subtilis spores used as display system. Both antigens where able to induce a specific immune response suggesting BclA2CTD and BclA3CTD as promising mucosal antigens for a vaccine targeting C. difficile spores. Plus, B. subtilis spores displaying CdeCCTD, another protein from C.difficile exosporium, were shown to adhere more efficiently to differentiated and undifferentiated Caco-2 than spores displaying the other antigens or wild type spores supporting the role of this protein in the interaction of C. difficile spores with the host and therefore in the initial steps of the C. difficile infection.


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