Sibilio, Sara
(2021)
Developing of an organ on chip device as novel in vitro platform to
study organ mechanobiology: Peristalsis on a chip.
[Tesi di dottorato]
Item Type: |
Tesi di dottorato
|
Lingua: |
English |
Title: |
Developing of an organ on chip device as novel in vitro platform to
study organ mechanobiology: Peristalsis on a chip. |
Creators: |
Creators | Email |
---|
Sibilio, Sara | saras.sibilio@gmail.com |
|
Date: |
15 June 2021 |
Number of Pages: |
128 |
Institution: |
Università degli Studi di Napoli Federico II |
Department: |
Ingegneria Chimica, dei Materiali e della Produzione Industriale |
Dottorato: |
Ingegneria dei prodotti e dei processi industriali |
Ciclo di dottorato: |
33 |
Coordinatore del Corso di dottorato: |
nome | email |
---|
D'Anna, Andrea | andrea.danna@unina.it |
|
Tutor: |
nome | email |
---|
Netti, Paolo Antonio | UNSPECIFIED |
|
Date: |
15 June 2021 |
Number of Pages: |
128 |
Uncontrolled Keywords: |
In-Crypts Device, Peristalsis, Peristalsis on a chip, patophysology |
Settori scientifico-disciplinari del MIUR: |
Area 09 - Ingegneria industriale e dell'informazione > ING-IND/06 - Fluidodinamica Area 09 - Ingegneria industriale e dell'informazione > ING-IND/34 - Bioingegneria industriale |
[error in script]
[error in script]
Date Deposited: |
20 Jul 2021 12:05 |
Last Modified: |
07 Jun 2023 11:08 |
URI: |
http://www.fedoa.unina.it/id/eprint/13773 |

Abstract
Developing of an organ on chip device as novel in vitro platform to study
organ mechanobiology: Peristalsis on a chip.
Knowing the mechanical properties of the gastrointestinal (GI) tract appears to be important for
understanding the molecular and cellular responses to mechanical stimuli on physiological
processes such as foods, xenobiotic or drugs digestion/absorption. These processes are
mediated by various intestinal cells such as epithelial cells, interstitial cells, smooth muscle
cells, and neurocytes. The loss or dysfunction of specific cells or mechanical strength of cell
bowel wall directly results in GI tract disease. Reversing the abnormal status of pathogenic cells
has been considered crucial to treatment of gut diseases. Gut bioengineered models have been
developing for the purpose to replace the damaged tissues and to provide three-dimensional
platforms that mimic the in vivo environment to study drug development, absorption and
toxicity. Nevertheless, the need to develop more complex models in vitro to study mechanical
stress is growing. In this perspective, this project will allow us to get an automatized
microfluidic gut platform to evaluate the pathophysiology of the small intestine through the
study of the shear stress of the bolus on the epithelial cells layer at the lumen side of the healthy
or diseased 3D intestine models. To this aim, the major goals of this project are the the design
and fabrication of complex and innovative microfluidic device provided with an integrated
PDMS membrane designed to mimic the crypt-villus axis in order to promote the differentiation
of the intestinal epithelium and the establishment of peristaltic motion by means of an
automatized and controlled elettrovalve system. The platform was used to estimate the intestinal
transport properties of the bolus and the physiological condition of the shear stress under
peristaltic motion. An important feature of the device, is the possibility to induce a fluid flow
both at the basolateral and the lumen side of the intestinal epithelium, therefore the possibility
to introduce integrated electrodes in the apical side and basoteral side in order to be enable
continuous monitoring of cells behaviour and differentiation through TransEpithelial Electrical
Resistance measurements. The effect of PDMS membrane morphology, peristaltic motion and
shear stress on intestinal epithelial cell differentiation, mucus production and molecules
adsorption process has been evaluated. The development of the Peristalsis on chip device could
be reduce the poorly predictive preclinical evaluation generated by the phylogenetic distance
between laboratory animals and humans, the discrepancy between current in vitro systems and
the human body, and the restrictions of in silico modelling.
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