D'Auria, Giovanni (2022) Proteomic characterization and definition of the potential allergenicity of sustainable novel food. [Tesi di dottorato]
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| Tipologia del documento: | Tesi di dottorato |
|---|---|
| Lingua: | English |
| Titolo: | Proteomic characterization and definition of the potential allergenicity of sustainable novel food |
| Autori: | Autore Email D'Auria, Giovanni giovanni.dauria@unina.it |
| Data: | 8 Marzo 2022 |
| Numero di pagine: | 221 |
| Istituzione: | Università degli Studi di Napoli Federico II |
| Dipartimento: | Agraria |
| Dottorato: | Scienze agrarie e agroalimentari |
| Ciclo di dottorato: | 34 |
| Coordinatore del Corso di dottorato: | nome email Barone, Amalia ambarone@unina.it |
| Tutor: | nome email Ferranti, Pasquale [non definito] Mamone, Gianfranco [non definito] |
| Data: | 8 Marzo 2022 |
| Numero di pagine: | 221 |
| Parole chiave: | Allergens, Proteomics, Novel-food |
| Settori scientifico-disciplinari del MIUR: | Area 07 - Scienze agrarie e veterinarie > AGR/15 - Scienze e tecnologie alimentari |
| Depositato il: | 18 Mar 2022 10:31 |
| Ultima modifica: | 28 Feb 2024 14:02 |
| URI: | http://www.fedoa.unina.it/id/eprint/14534 |
Abstract
The objectives of the present PhD project are summarized as follow: A1) evaluation of the preliminary allergenicity potential of Moringa oleifera leaves through proteomic(SDS-PAGE, RP-HPLC, LC- HR-MS/MS), in-silico and in-vitro approaches; A2) Determination of the proteome of an innovative hybrid crop (tritordeum) by using RP-HPLC and gel-based proteomic techniques. The product of in-vitro digestion (INFOGEST) of tritordeum flours and bread were evaluated in-silico and in-vitro for their potential immunoreactivity (celiacogenic and allergenic potential). A3) In-vitro digestion (INFOGEST) and evaluation through proteomic and peptidomic approaches (MALDI-TOF LC-MS/MS) of the fermentation impact on the allergenic potential and the possible bioactivity of a fermented dairy by-product (kashk). The bioactivity of kashk extracts was also evaluated in-vitro.
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