Andretta, Emanuela (2023) Evaluation of capsaicin anticancer properties on mesothelioma. [Tesi di dottorato]
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| Item Type: | Tesi di dottorato |
|---|---|
| Resource language: | English |
| Title: | Evaluation of capsaicin anticancer properties on mesothelioma |
| Creators: | Creators Email Andretta, Emanuela emanuela.andretta@unina.it |
| Date: | 3 March 2023 |
| Number of Pages: | 76 |
| Institution: | Università degli Studi di Napoli Federico II |
| Department: | Medicina Veterinaria e Produzioni Animali |
| Dottorato: | Scienze veterinarie |
| Ciclo di dottorato: | 35 |
| Coordinatore del Corso di dottorato: | nome email De Girolamo, Paolo paolo.degirolamo@unina.it |
| Tutor: | nome email Ciarcia, Roberto UNSPECIFIED |
| Date: | 3 March 2023 |
| Number of Pages: | 76 |
| Keywords: | Mesothelioma, Capsaicin, AKT, ERK1/2, migration, proliferation, cell cycle, cisplatin-resistance, autophagy. |
| Settori scientifico-disciplinari del MIUR: | Area 07 - Scienze agrarie e veterinarie > VET/07 - Farmacologia e tossicologia veterinaria |
| Additional information: | indirizzo di residenza: via Fratelli Cervi n°108, isolato i scala A, Napoli, 80144 altra mail: emanuelaandretta94@gmail.com email unina: emanuela.andretta@unina.it telefono fisso: 0817011220 cellulare: 3394887655 |
| Date Deposited: | 17 Mar 2023 11:42 |
| Last Modified: | 10 Apr 2025 14:12 |
| URI: | http://www.fedoa.unina.it/id/eprint/15212 |
Collection description
ABSTRACT Background: Mesothelioma is an aggressive cancer with limited treatment options. Capsaicin (CAPS) is a compound known for its antitumor properties on several cancer lines. Anywaway, the effect of CAPS on proliferation and migration of mesothelioma cells needs to be investigated. Methods: The effect of CAPS on the proliferation of several mesothelioma cells, representative of the main subtypes of mesothelioma, was evaluated by MTS assay. The ratio of phosphorylated and total form of both AKT and ERK1/2 and the levels of markers involved in apoptosis and autophagy were evaluated by western blotting. Furthermore, wound healing assays and transwell migration assays were performed to assess the anti-migration action of CAPS on different mesothelioma cell lines. Results: In the present study we demonstrate that CAPS inhibits cell growth of both parental and cisplatin-resistant mesothelioma cells. CAPS exerts an antiproliferative action by disrupting the cell cycle and inducing S-phase arrest and reduces lateral motility and migration in various mesothelioma cell lines. Furthermore, CAPS treatment suppressed AKT and ERK1/2 activation in both MSTO-211H and NCI-H2052 cells. Preliminary results show that CAPS can impair the expression of markers involved in the autophagy process, such as LC3A/B II/I ratio and Beclin-1 level. Conclusions: Our results demonstrate the antiproliferative and anti-migratory effect of CAPS on various mesothelioma cells. Since CAPS also reduces the proliferation of cisplatin-resistant mesothelioma cells, CAPS may represent a promising strategy to overcome drug-resistance and increase drug response in mesothelioma cells. Given the encouraging results obtained from this work, further studies are needed to evaluate the possible use of CAPS in the treatment of mesothelioma.
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