Borbone, Eleonora (2009) EVALUATION OF HISTONE DEACETYLASE INHIBITOR EFFECTS ON THYROID CANCER. [Tesi di dottorato] (Unpublished)

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Item Type: Tesi di dottorato
Language: English
Title: EVALUATION OF HISTONE DEACETYLASE INHIBITOR EFFECTS ON THYROID CANCER
Creators:
CreatorsEmail
Borbone, Eleonoraelly_norab@yahoo.it
Date: 31 March 2009
Number of Pages: 89
Institution: Università degli Studi di Napoli Federico II
Department: CEINGE Biotecnologie avanzate
Doctoral School: Scuola Europea di Medicina Molecolare - SEMM Sede di Napoli
PHD name: Phd in Molecular Medicine, Curriculum Molecular Oncology
PHD cycle: 20
PHD Coordinator:
nameemail
Salvatore, FrancescoUNSPECIFIED
Tutor:
nameemail
Fusco, Alfredoafusco@napoli.com
Simeone, AntonioUNSPECIFIED
Gronemeyer, HinrichUNSPECIFIED
Date: 31 March 2009
Number of Pages: 89
Uncontrolled Keywords: HDAC, thyroid, cancer
MIUR S.S.D.: Area 05 - Scienze biologiche > BIO/11 - Biologia molecolare
Area 06 - Scienze mediche > MED/06 - Oncologia medica
Date Deposited: 16 Nov 2009 14:59
Last Modified: 30 Apr 2014 19:36
URI: http://www.fedoa.unina.it/id/eprint/3402

Abstract

Abstract Histone deacetylases (HDACs) play a crucial role in the proper regulation of cellular functions through their connection with chromatin and transcriptional regulation. Alterations in HDAC activity have been reported in several types of cancer encouraging development of HDAC inhibitors (HDACis) for cancer treatment. The antitumor activity of HDACi has been demonstrated, in clinical trials, in both solid and non solid neoplasias at doses well tolerated by patients. However, the molecular basis for their tumor selectivity is unknown. Anaplastic thyroid carcinoma (ATC) is one of the most aggressive malignancies, having a poor prognosis and being refractory to conventional chemo- and radiotherapy. To the aim to find an innovative therapy for the treatment of ATCs, we studied the effects of two potent HDACis, SAHA and MS-275, on rat thyroid cell lines transformed by the v-ras-Ki oncogene which is frequently mutated in ATCs. We show that: i) HDAC 1 and HDAC 2 are overexpressed in anaplastic thyroid carcinomas compared to normal thyroid; ii) SAHA and MS-275 induce apoptosis selectively in completely transformed rat thyroid cells; iii) TNF-related apoptosis-inducing ligand (TRAIL) is the main mediator of cell death induced by SAHA; iv) SAHA stabilize TRAIL protein by affecting its proteasome-mediated degradation.

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