Scialdone, Antonio (2010) Statistical mechanics of genome regulation: the case of X chromosome inactivation. [Tesi di dottorato] (Unpublished)

[img]
Preview
PDF
scialdone_antonio_23.pdf

Download (10MB) | Preview

Abstract

The aim of my PhD research project was to discover the mechanisms behind X Chromosome Inactivation (XCI) one of the most intriguing issues of the current mammalian Biology. XCI is the process whereby a female mammal cell silences one of its two X chromosomes randomly chosen, to equalize the dosage of X products with respect to males (having just one X). We used theoretical models from Statistical Physics and their massive computer simulations to dissect this chromosome-wide stochastic regulatory process. The importance of these investigations goes beyond the XCI, as the comprehension of this process, can indeed shed light on a whole class of regulatory mechanisms involving the genome. By means of our quantitive models, which already found some important experimental confirmations, we were able to provide a new deeper level of understanding of the underlying physical and molecular mechanisms. Precise predictions are given for many genetic/chemical manipulations and a new generation of experiments can be designed. A close interplay between theory and experiments, was guaranteed in our project by the collaboration with an experimental group from Harvard Medical School, USA.

Item Type: Tesi di dottorato
Uncontrolled Keywords: Statistical Mechanics; genome regulation; X inactivation; DNA spatial architecture; stochastic regulatory mechanisms
Depositing User: Francesca Migliorini
Date Deposited: 08 Dec 2010 12:01
Last Modified: 30 Apr 2014 19:43
URI: http://www.fedoa.unina.it/id/eprint/7951

Actions (login required)

View Item View Item