Di Caprio, Giuseppe (2011) Quantitative label-free cell imaging by means of digital holographic miscroscopy: a roadmap for a complete characterization of biological samples. [Tesi di dottorato] (Unpublished)

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Item Type: Tesi di dottorato
Lingua: English
Title: Quantitative label-free cell imaging by means of digital holographic miscroscopy: a roadmap for a complete characterization of biological samples
Creators:
CreatorsEmail
Di Caprio, Giuseppegiuseppe.dicaprio@na.imm.cnr.it
Date: 30 November 2011
Number of Pages: 106
Institution: Università degli Studi di Napoli Federico II
Department: Scienze fisiche
Scuola di dottorato: Ingegneria industriale
Dottorato: Tecnologie innovative per materiali, sensori ed imaging
Ciclo di dottorato: 24
Coordinatore del Corso di dottorato:
nomeemail
Andreone, Antonelloandreone@unina.it
Tutor:
nomeemail
Coppola, Giuseppegiuseppe.coppola@cnr.it
De Stefano, Lucaluca.destefano@cnr.it
Date: 30 November 2011
Number of Pages: 106
Uncontrolled Keywords: Digital holografic microscopy; label-free technique; Morphology; tracking; sperm cells; fertility; microfluidics; phase analysis;
Settori scientifico-disciplinari del MIUR: Area 02 - Scienze fisiche > FIS/03 - Fisica della materia
Additional Information: The research activity has been performed at the institute for Microelectronic and Microsystems - National Council of Research.
Date Deposited: 12 Dec 2011 12:29
Last Modified: 30 Apr 2014 19:47
URI: http://www.fedoa.unina.it/id/eprint/8708
DOI: 10.6092/UNINA/FEDOA/8708

Abstract

The research activity, performed at the institute for Microelectronic and Microsystems, has been devoted to the study and the development of non-destructive optical techniques for morphological analysis of biological samples. A digital holographic microscope has been designed and set up, compatible with micro-manufactured devices, for a label-free, non-destructive characterization of biological samples, as particular regard to bovine and human spermatozoa. Moreover, dedicated algorithms have been developed in order to elaborate the acquired hologram and to retrieve the needed biological parameters of the analyzed sample.

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