Di Caprio, Giuseppe (2011) Quantitative label-free cell imaging by means of digital holographic miscroscopy: a roadmap for a complete characterization of biological samples. [Tesi di dottorato] (Unpublished)

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Item Type: Tesi di dottorato
Language: English
Title: Quantitative label-free cell imaging by means of digital holographic miscroscopy: a roadmap for a complete characterization of biological samples
Creators:
CreatorsEmail
Di Caprio, Giuseppegiuseppe.dicaprio@na.imm.cnr.it
Date: 30 November 2011
Number of Pages: 106
Institution: Università degli Studi di Napoli Federico II
Department: Scienze fisiche
Doctoral School: Ingegneria industriale
PHD name: Tecnologie innovative per materiali, sensori ed imaging
PHD cycle: 24
PHD Coordinator:
nameemail
Andreone, Antonelloandreone@unina.it
Tutor:
nameemail
Coppola, Giuseppegiuseppe.coppola@cnr.it
De Stefano, Lucaluca.destefano@cnr.it
Date: 30 November 2011
Number of Pages: 106
Uncontrolled Keywords: Digital holografic microscopy; label-free technique; Morphology; tracking; sperm cells; fertility; microfluidics; phase analysis;
MIUR S.S.D.: Area 02 - Scienze fisiche > FIS/03 - Fisica della materia
Additional Information: The research activity has been performed at the institute for Microelectronic and Microsystems - National Council of Research.
Date Deposited: 12 Dec 2011 12:29
Last Modified: 30 Apr 2014 19:47
URI: http://www.fedoa.unina.it/id/eprint/8708

Abstract

The research activity, performed at the institute for Microelectronic and Microsystems, has been devoted to the study and the development of non-destructive optical techniques for morphological analysis of biological samples. A digital holographic microscope has been designed and set up, compatible with micro-manufactured devices, for a label-free, non-destructive characterization of biological samples, as particular regard to bovine and human spermatozoa. Moreover, dedicated algorithms have been developed in order to elaborate the acquired hologram and to retrieve the needed biological parameters of the analyzed sample.

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