Di Caprio, Giuseppe (2011) Quantitative label-free cell imaging by means of digital holographic miscroscopy: a roadmap for a complete characterization of biological samples. [Tesi di dottorato] (Inedito)
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| Tipologia del documento: | Tesi di dottorato |
|---|---|
| Lingua: | English |
| Titolo: | Quantitative label-free cell imaging by means of digital holographic miscroscopy: a roadmap for a complete characterization of biological samples |
| Autori: | Autore Email Di Caprio, Giuseppe giuseppe.dicaprio@na.imm.cnr.it |
| Data: | 30 Novembre 2011 |
| Numero di pagine: | 106 |
| Istituzione: | Università degli Studi di Napoli Federico II |
| Dipartimento: | Scienze fisiche |
| Scuola di dottorato: | Ingegneria industriale |
| Dottorato: | Tecnologie innovative per materiali, sensori ed imaging |
| Ciclo di dottorato: | 24 |
| Coordinatore del Corso di dottorato: | nome email Andreone, Antonello andreone@unina.it |
| Tutor: | nome email Coppola, Giuseppe giuseppe.coppola@cnr.it De Stefano, Luca luca.destefano@cnr.it |
| Data: | 30 Novembre 2011 |
| Numero di pagine: | 106 |
| Parole chiave: | Digital holografic microscopy; label-free technique; Morphology; tracking; sperm cells; fertility; microfluidics; phase analysis; |
| Settori scientifico-disciplinari del MIUR: | Area 02 - Scienze fisiche > FIS/03 - Fisica della materia |
| Informazioni aggiuntive: | The research activity has been performed at the institute for Microelectronic and Microsystems - National Council of Research. |
| Depositato il: | 12 Dic 2011 12:29 |
| Ultima modifica: | 30 Apr 2014 19:47 |
| URI: | http://www.fedoa.unina.it/id/eprint/8708 |
| DOI: | 10.6092/UNINA/FEDOA/8708 |
Abstract
The research activity, performed at the institute for Microelectronic and Microsystems, has been devoted to the study and the development of non-destructive optical techniques for morphological analysis of biological samples. A digital holographic microscope has been designed and set up, compatible with micro-manufactured devices, for a label-free, non-destructive characterization of biological samples, as particular regard to bovine and human spermatozoa. Moreover, dedicated algorithms have been developed in order to elaborate the acquired hologram and to retrieve the needed biological parameters of the analyzed sample.
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