Trotta, Roberta (2011) Insight into macromolecular interactions involving nucleic acids: a study of the interaction of G-quadruplexes with pharmacologically active molecules and investigations of the RNA binding properties of LARP4 protein. [Tesi di dottorato] (Unpublished)

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Item Type: Tesi di dottorato
Language: English
Title: Insight into macromolecular interactions involving nucleic acids: a study of the interaction of G-quadruplexes with pharmacologically active molecules and investigations of the RNA binding properties of LARP4 protein.
Creators:
CreatorsEmail
Trotta, Robertaroberta.trotta@unina.it
Date: 30 November 2011
Number of Pages: 198
Institution: Università degli Studi di Napoli Federico II
Department: Chimica delle sostanze naturali
Doctoral School: Scienze farmaceutiche
PHD name: Scienza del farmaco
PHD cycle: 24
PHD Coordinator:
nameemail
D'Auria, Maria Valeriamadauria@unina.it
Tutor:
nameemail
Randazzo, Antonioantonio.randazzo@unina.it
Date: 30 November 2011
Number of Pages: 198
Uncontrolled Keywords: G-quadruplex;NMR;LARP4 protein
MIUR S.S.D.: Area 03 - Scienze chimiche > CHIM/06 - Chimica organica
Area 05 - Scienze biologiche > BIO/11 - Biologia molecolare
Area 03 - Scienze chimiche > CHIM/08 - Chimica farmaceutica
Date Deposited: 06 Dec 2011 11:35
Last Modified: 26 Sep 2014 10:32
URI: http://www.fedoa.unina.it/id/eprint/8950

Abstract

This dissertation can be divided in two parts. In the first part, my efforts have been focused on the study of the Gquadruplex structures. In chapter one, I describe the unusual arrangement of the DNA to form the G-quadruplex structures and their biological role, underlying the reason why in the last period a lot of research groups focused their studies on this topic. In chapter two, after a brief introduction of the first molecules capable to bind the G-quadruplex (Distamicyn A and its derivates), I describe the new organic molecule capable to interact with the DNA quadruplex, which I have discovered applying some innovative approaches during my PhD. In the second part of this thesis I introduce the research conducted in the laboratory of Dr. Maria R. Conte at the King’s College of London. In this stimulating environment I started studying the structure of the protein LARP4 and its interaction with a poly (A) RNA using the NMR. In the course of my research I have learnt the techniques allowing to do the transformation, the expression and the purification of the protein. A summary presentation of the techniques I have applied is reported in the second part in chapter five.

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