Trotta, Roberta (2011) Insight into macromolecular interactions involving nucleic acids: a study of the interaction of G-quadruplexes with pharmacologically active molecules and investigations of the RNA binding properties of LARP4 protein. [Tesi di dottorato] (Inedito)

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Tipologia del documento: Tesi di dottorato
Lingua: English
Titolo: Insight into macromolecular interactions involving nucleic acids: a study of the interaction of G-quadruplexes with pharmacologically active molecules and investigations of the RNA binding properties of LARP4 protein.
Autori:
AutoreEmail
Trotta, Robertaroberta.trotta@unina.it
Data: 30 Novembre 2011
Numero di pagine: 198
Istituzione: Università degli Studi di Napoli Federico II
Dipartimento: Chimica delle sostanze naturali
Scuola di dottorato: Scienze farmaceutiche
Dottorato: Scienza del farmaco
Ciclo di dottorato: 24
Coordinatore del Corso di dottorato:
nomeemail
D'Auria, Maria Valeriamadauria@unina.it
Tutor:
nomeemail
Randazzo, Antonioantonio.randazzo@unina.it
Data: 30 Novembre 2011
Numero di pagine: 198
Parole chiave: G-quadruplex;NMR;LARP4 protein
Settori scientifico-disciplinari del MIUR: Area 03 - Scienze chimiche > CHIM/06 - Chimica organica
Area 05 - Scienze biologiche > BIO/11 - Biologia molecolare
Area 03 - Scienze chimiche > CHIM/08 - Chimica farmaceutica
Depositato il: 06 Dic 2011 11:35
Ultima modifica: 15 Lug 2015 01:00
URI: http://www.fedoa.unina.it/id/eprint/8950

Abstract

This dissertation can be divided in two parts. In the first part, my efforts have been focused on the study of the Gquadruplex structures. In chapter one, I describe the unusual arrangement of the DNA to form the G-quadruplex structures and their biological role, underlying the reason why in the last period a lot of research groups focused their studies on this topic. In chapter two, after a brief introduction of the first molecules capable to bind the G-quadruplex (Distamicyn A and its derivates), I describe the new organic molecule capable to interact with the DNA quadruplex, which I have discovered applying some innovative approaches during my PhD. In the second part of this thesis I introduce the research conducted in the laboratory of Dr. Maria R. Conte at the King’s College of London. In this stimulating environment I started studying the structure of the protein LARP4 and its interaction with a poly (A) RNA using the NMR. In the course of my research I have learnt the techniques allowing to do the transformation, the expression and the purification of the protein. A summary presentation of the techniques I have applied is reported in the second part in chapter five.

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