Accardo, Maria Carmela (2006) Un’analisi genomica per l’identificazione di nuovi geni che codificano piccoli RNA nucleolari in Drosophila melanogaster. [Tesi di dottorato] (Unpublished)

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Item Type: Tesi di dottorato
Language: Italiano
Title: Un’analisi genomica per l’identificazione di nuovi geni che codificano piccoli RNA nucleolari in Drosophila melanogaster
Creators:
CreatorsEmail
Accardo, Maria CarmelaUNSPECIFIED
Date: 2006
Date Type: Publication
Number of Pages: 237
Institution: Università degli Studi di Napoli Federico II
Department: Biologia e patologia cellullare e molecolare "L. Califano"
PHD name: Genetica e medicina molecolare
PHD cycle: 17
PHD Coordinator:
nameemail
Bruni, Carmelo BrunoUNSPECIFIED
Tutor:
nameemail
Furia, MariaUNSPECIFIED
Date: 2006
Number of Pages: 237
Uncontrolled Keywords: Drosophila melanogaster, ncRNAs, snoRNAs, rRNAs, 2-O’- ribose methylation
MIUR S.S.D.: Area 05 - Scienze biologiche > BIO/11 - Biologia molecolare
Area 06 - Scienze mediche > MED/03 - Genetica medica
Area 05 - Scienze biologiche > BIO/13 - Biologia applicata
Area 06 - Scienze mediche > MED/07 - Microbiologia e microbiologia clinica
Area 05 - Scienze biologiche > BIO/12 - Biochimica clinica e biologia molecolare clinica
Area 05 - Scienze biologiche > BIO/18 - Genetica
Date Deposited: 25 Jul 2008
Last Modified: 30 Apr 2014 19:24
URI: http://www.fedoa.unina.it/id/eprint/912

Abstract

In eukaryotes, the family of non-coding RNA genes includes a number of genes encoding small nucleolar RNAs (mainly of the C/D and H/ACA families), which act as guides in the maturation or post-trascriptional modifications of target RNA molecules. We have performed a genome-wide analysis of Drosophila melanogaster genome searching for C/D snoRNA genes by a computational approach based on the use of the SNOSCAN program and the methylation sites conserved between yeast, human and Drosophila rRNAs, followed by experimental validation of the putative candidates. This analysis led to confirm the expression of 52 out of the 100 putative snoRNA genes identified by bioinformatic analysis. Organization of the Dm genome was found to be more variegated than previously suspected, with snoRNA genes nested in both the introns and the exons of protein-coding genes. This finding suggests that the presence of additional mechanisms of snoRNA biogenesis based on the alternative production of overlapping mRNA/snoRNA molecules. Interestingly, this analysis led to identify not only snoRNAs able to target rRNA but also at least six molecules able to target snRNAs, while nine novel snoRNAs were classified as “orphans”. Searching in the Drosophila transcriptome for potential additional targets, we found that many mRNAs may potentially be modified by these orphan molecules.

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