Accardo, Maria Carmela (2006) Un’analisi genomica per l’identificazione di nuovi geni che codificano piccoli RNA nucleolari in Drosophila melanogaster. [Tesi di dottorato] (Inedito)
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| Tipologia del documento: | Tesi di dottorato | ||||
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| Lingua: | Italiano | ||||
| Titolo: | Un’analisi genomica per l’identificazione di nuovi geni che codificano piccoli RNA nucleolari in Drosophila melanogaster | ||||
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| Data: | 2006 | ||||
| Tipo di data: | Pubblicazione | ||||
| Numero di pagine: | 237 | ||||
| Istituzione: | Università degli Studi di Napoli Federico II | ||||
| Dipartimento: | Biologia e patologia cellullare e molecolare "L. Califano" | ||||
| Dottorato: | Genetica e medicina molecolare | ||||
| Ciclo di dottorato: | 17 | ||||
| Coordinatore del Corso di dottorato: |
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| Tutor: |
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| Data: | 2006 | ||||
| Numero di pagine: | 237 | ||||
| Parole chiave: | Drosophila melanogaster, ncRNAs, snoRNAs, rRNAs, 2-O’- ribose methylation | ||||
| Settori scientifico-disciplinari del MIUR: | Area 05 - Scienze biologiche > BIO/11 - Biologia molecolare Area 06 - Scienze mediche > MED/03 - Genetica medica Area 05 - Scienze biologiche > BIO/13 - Biologia applicata Area 06 - Scienze mediche > MED/07 - Microbiologia e microbiologia clinica Area 05 - Scienze biologiche > BIO/12 - Biochimica clinica e biologia molecolare clinica Area 05 - Scienze biologiche > BIO/18 - Genetica |
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| Depositato il: | 25 Lug 2008 | ||||
| Ultima modifica: | 30 Apr 2014 19:24 | ||||
| URI: | http://www.fedoa.unina.it/id/eprint/912 | ||||
| DOI: | 10.6092/UNINA/FEDOA/912 |
Abstract
In eukaryotes, the family of non-coding RNA genes includes a number of genes encoding small nucleolar RNAs (mainly of the C/D and H/ACA families), which act as guides in the maturation or post-trascriptional modifications of target RNA molecules. We have performed a genome-wide analysis of Drosophila melanogaster genome searching for C/D snoRNA genes by a computational approach based on the use of the SNOSCAN program and the methylation sites conserved between yeast, human and Drosophila rRNAs, followed by experimental validation of the putative candidates. This analysis led to confirm the expression of 52 out of the 100 putative snoRNA genes identified by bioinformatic analysis. Organization of the Dm genome was found to be more variegated than previously suspected, with snoRNA genes nested in both the introns and the exons of protein-coding genes. This finding suggests that the presence of additional mechanisms of snoRNA biogenesis based on the alternative production of overlapping mRNA/snoRNA molecules. Interestingly, this analysis led to identify not only snoRNAs able to target rRNA but also at least six molecules able to target snRNAs, while nine novel snoRNAs were classified as “orphans”. Searching in the Drosophila transcriptome for potential additional targets, we found that many mRNAs may potentially be modified by these orphan molecules.
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