Eid, Ahmed (2013) Biological control of post-harvest diseases on apple by using plant essential oils and Trichoderma culture filtrates. [Tesi di dottorato]

Ahmed Eid (PhD Thesis)-A (2).pdf

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Item Type: Tesi di dottorato
Lingua: English
Title: Biological control of post-harvest diseases on apple by using plant essential oils and Trichoderma culture filtrates
Eid, Ahmedeltabeyaid@mans.edu.eg, eltabeyaid@yahoo.com
Date: 2 April 2013
Number of Pages: 69
Institution: Università degli Studi di Napoli Federico II
Department: Agraria
Scuola di dottorato: Scienze agrarie e agro-alimentari
Dottorato: Agrobiologia e agrochimica
Ciclo di dottorato: 25
Coordinatore del Corso di dottorato:
Lorito, Matteolorito@unina.it
Coordinatore del Corso di dottorato (extra):
Lorito, Matteolorito@unina.it
Ruocco, Michelinamiruocco@unina.it
Date: 2 April 2013
Number of Pages: 69
Uncontrolled Keywords: Biological control, apple post-harvest diseases, essential oils, Trichoderma CF, bio-fungicide formulation
Settori scientifico-disciplinari del MIUR: Area 07 - Scienze agrarie e veterinarie > AGR/12 - Patologia vegetale
Additional Information: -
Date Deposited: 04 Apr 2013 13:13
Last Modified: 22 Sep 2014 08:14
URI: http://www.fedoa.unina.it/id/eprint/9436
DOI: 10.6092/UNINA/FEDOA/9436


Pure essential oils have been purified and chemically characterized from cinnamon, clove and black pepper. All these oils have antifungal properties and when applied to apple fruits in post harvest can efficiently control Botrytis cinerea, Alternaria alternata or Penicillium expansum. In addition, application of these compounds or Trichoderma culture filtrate can induce systemic resistance mechanisms in apple fruit. This has been confirmed through phytoalexins and proteomic analysis. The accumulation of the phytoalexins scopoletin, umbelliferone, and scoparone was induced by the three essential oils or Trichoderma CF. Proteomic analysis indicated many changes in protein production caused by the treatment with the oils, Trichoderma CF and the pathogens. The differential spots (TOTD) obtained were up to 166 in clove plus P. expansum treatment. Twenty nine of the most interesting differentially expressed proteins were further analysed by MALDI-TOF MS. The protein groups identified included: pathogenesis-related proteins belonging to the PR-10 sub-family, some antimicrobial proteins, enzymes involved in the biosynthesis of antimicrobial compounds pathway and ribosomal proteins. The most interesting essential oil was the one extracted from black pepper because it was not phytotoxic on tomato seedlings and apple fruits at all the tested concentrations and showed a good disease control activity. A new bio-formulate for post-harvest application, based on a combination of three different active ingredients (black pepper oil, Trichoderma CF and a natural ISR-inducing agent also used as emulsifier) was designed and successfully tested as a synergistic mixture.


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