Vitale, Monica (2013) Characterization of Carbonic Anhydrases in Models of Neuronal Differentiation and in the Nematode Caenorhabditis elegans. [Tesi di dottorato]

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Item Type: Tesi di dottorato
Lingua: English
Title: Characterization of Carbonic Anhydrases in Models of Neuronal Differentiation and in the Nematode Caenorhabditis elegans
Creators:
CreatorsEmail
Vitale, Monicamonica.vitale2@unina.it
Date: 2 April 2013
Number of Pages: 86
Institution: Università degli Studi di Napoli Federico II
Department: Medicina Molecolare e Biotecnologie Mediche
Scuola di dottorato: Medicina molecolare
Dottorato: Neuroscienze
Ciclo di dottorato: 25
Coordinatore del Corso di dottorato:
nomeemail
Annunziato, Luciolannunziato@unina.it
Tutor:
nomeemail
Zambrano, Nicolazambrano@unina.it
Date: 2 April 2013
Number of Pages: 86
Uncontrolled Keywords: Hypoxia, nucleocytoplasmatic transport, ARM/HEAT repeats
Settori scientifico-disciplinari del MIUR: Area 05 - Scienze biologiche > BIO/11 - Biologia molecolare
Date Deposited: 10 Apr 2013 09:20
Last Modified: 15 Jul 2015 01:00
URI: http://www.fedoa.unina.it/id/eprint/9515
DOI: 10.6092/UNINA/FEDOA/9515

Abstract

CA IX is a member of the carbonic anhydrase family of enzymes. It is a well known marker of hypoxia and it is involved in pH and survival regulation in hypoxic cells. The main aim of my PhD project was to identify molecular interactors of CA IX and, based on the knowledge of its ligands, to contribute to functional characterization of CA IX in neuronal cells. A complex protein network of novel CA IX interactors has been highlighted: several proteins of the nucleo-cytoplasmatic machinery have been found to bind CA IX under hypoxic condition; many of the CA IX protein interactors belong to the family of the ARM and HEAT-repeat containing proteins. Both in normoxic and hypoxic conditions CA IX also interacts with Cullin-associated NEDD8-dissociated protein 1 (CAND1), which is a nuclear HEAT/ARM-containing protein that is involved in gene transcription and assembly of the SCF E3 ubiquitine ligase complexes. Immunofluorescence (IF) analysis demonstrated an accumulation of CA IX in the nuclei of neuroblastoma cell lines and in neurons derived from murine ESCs. Putative NLS/NES sequences were identified in the CA IX protein sequence; IF analysis showed that they were able to affect distribution of the reporter protein GFP inside the cell. Collectively, these data suggest that subcellular localization and functions of CA IX are more complex than previously thought. CA IX may have intracellular functions different from those already known at the plasma membrane. A nuclear function for CA IX is in fact suggested by its localization on transcribed chromatin. Finally, the nematode Caenorhabditis elegans was used as an animal model, in order to characterize the function of two carbonic anhydrases of the worm, namely, cah-5 and cah-6, during hypoxic and anoxic stresses. Although preliminary, the observed phenotypes allow to predict fundamental roles for carbonic anhydrases in vivo.

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