Bagattini, Maria (2006) Epidemiologia molecolare di Klebsiella pneumoniae produttore di beta-lattamasi ad ampio spettro (ESBL) circolante in una Terapia Intensiva Neonatale. [Tesi di dottorato] (Unpublished)
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| Item Type: | Tesi di dottorato |
|---|---|
| Resource language: | Italiano |
| Title: | Epidemiologia molecolare di Klebsiella pneumoniae produttore di beta-lattamasi ad ampio spettro (ESBL) circolante in una Terapia Intensiva Neonatale |
| Creators: | Creators Email Bagattini, Maria UNSPECIFIED |
| Date: | 2006 |
| Date type: | Publication |
| Number of Pages: | 41 |
| Institution: | Università degli Studi di Napoli Federico II |
| Department: | Scienze mediche preventive |
| Dottorato: | Ambiente, prevenzione e medicina pubblica |
| Ciclo di dottorato: | 17 |
| Coordinatore del Corso di dottorato: | nome email Buccelli, Claudio UNSPECIFIED |
| Tutor: | nome email Triassi, Maria UNSPECIFIED |
| Date: | 2006 |
| Number of Pages: | 41 |
| Keywords: | Klebsiella pneumoniae, ESBL (Extended-Spectrum Beta-Lactamase), Nosocomial infections |
| Settori scientifico-disciplinari del MIUR: | Area 06 - Scienze mediche > MED/43 - Medicina legale |
| Date Deposited: | 30 Jul 2008 |
| Last Modified: | 30 Apr 2014 19:24 |
| URI: | http://www.fedoa.unina.it/id/eprint/975 |
| DOI: | 10.6092/UNINA/FEDOA/975 |
Collection description
The molecular epidemiology of extended-spectrum beta-lactamase (ESBL)-producing Klebsiella pneumoniae was investigated in the neonatal intensive care unit (NICU) of a university hospital in Italy from September 2002 to December 2004, when 233 colonizations and 19 infections by K. pneumoniae occurred. Molecular typing by pulsed- field gel electrophoresis (PFGE) and dendrogram analysis of ESBL-producing K. pneumoniae isolates identified two distinct PFGE patterns B and C, that were sequentially isolated and that differed from one epidemic clone of PFGE type A isolated during 1996 in the same ward. Antimicrobial susceptibility patterns of ESBL-producing K. pneumoniae epidemic clones of PFGE type B and C showed an identical antibiotype, that differed from clone of PFGE type A for gentamicin resistance. DNA sequencing of amplified blaTEM and blaSHV genes resulted in the detection of a novel blaTEM ESBL gene, blaTEM-136, along with blaSHV-1 gene, in chromosomal and plasmid DNA from K. pneumoniae of PFGE type A, respectively, and blaTEM-1 and blaSHV-12 genes in chromosomal and plasmid DNA from K. pneumoniae epidemic clone of PFGE type B and C, respectively. Conjugation experiments demonstrated that resistance to third generation cephems, along with blaSHV-12 gene, was transferred from K. pneumoniae epidemic strains of PFGE type B and C to a susceptible E. coli host at a frequency of 4x 10-6 and 1 x 10-6 CFU/recipient cells, respectively. Our data suggest that the selection of ESBL producing clones and the transfer of blaSHV-12 ESBL gene between different clones were responsible for the spread of K. pneumoniae in the NICU.
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