Bagattini, Maria
(2006)
Epidemiologia molecolare di Klebsiella pneumoniae produttore di beta-lattamasi ad ampio spettro (ESBL) circolante in una Terapia Intensiva Neonatale.
[Tesi di dottorato]
(Unpublished)
| Item Type: |
Tesi di dottorato
|
| Lingua: |
Italiano |
| Title: |
Epidemiologia molecolare di Klebsiella pneumoniae produttore di beta-lattamasi ad ampio spettro (ESBL) circolante in una Terapia Intensiva Neonatale |
| Creators: |
| Creators | Email |
|---|
| Bagattini, Maria | UNSPECIFIED |
|
| Date: |
2006 |
| Date Type: |
Publication |
| Number of Pages: |
41 |
| Institution: |
Università degli Studi di Napoli Federico II |
| Department: |
Scienze mediche preventive |
| Dottorato: |
Ambiente, prevenzione e medicina pubblica |
| Ciclo di dottorato: |
17 |
| Coordinatore del Corso di dottorato: |
| nome | email |
|---|
| Buccelli, Claudio | UNSPECIFIED |
|
| Tutor: |
| nome | email |
|---|
| Triassi, Maria | UNSPECIFIED |
|
| Date: |
2006 |
| Number of Pages: |
41 |
| Uncontrolled Keywords: |
Klebsiella pneumoniae, ESBL (Extended-Spectrum Beta-Lactamase), Nosocomial infections |
| Settori scientifico-disciplinari del MIUR: |
Area 06 - Scienze mediche > MED/43 - Medicina legale |
[error in script]
[error in script]
| Date Deposited: |
30 Jul 2008 |
| Last Modified: |
30 Apr 2014 19:24 |
| URI: |
http://www.fedoa.unina.it/id/eprint/975 |
| DOI: |
10.6092/UNINA/FEDOA/975 |
Abstract
The molecular epidemiology of extended-spectrum beta-lactamase (ESBL)-producing Klebsiella pneumoniae was investigated in the neonatal intensive care unit (NICU) of a university hospital in Italy from September 2002 to December 2004, when 233 colonizations and 19 infections by K. pneumoniae occurred. Molecular typing by pulsed- field gel electrophoresis (PFGE) and dendrogram analysis of ESBL-producing K. pneumoniae isolates identified two distinct PFGE patterns B and C, that were sequentially isolated and that differed from one epidemic clone of PFGE type A isolated during 1996 in the same ward. Antimicrobial susceptibility patterns of ESBL-producing K. pneumoniae epidemic clones of PFGE type B and C showed an identical antibiotype, that differed from clone of PFGE type A for gentamicin resistance. DNA sequencing of amplified blaTEM and blaSHV genes resulted in the detection of a novel blaTEM ESBL gene, blaTEM-136, along with blaSHV-1 gene, in chromosomal and plasmid DNA from K. pneumoniae of PFGE type A, respectively, and blaTEM-1 and blaSHV-12 genes in chromosomal and plasmid DNA from K. pneumoniae epidemic clone of PFGE type B and C, respectively. Conjugation experiments demonstrated that resistance to third generation cephems, along with blaSHV-12 gene, was transferred from K. pneumoniae epidemic strains of PFGE type B and C to a susceptible E. coli host at a frequency of 4x 10-6 and 1 x 10-6 CFU/recipient cells, respectively. Our data suggest that the selection of ESBL producing clones and the transfer of blaSHV-12 ESBL gene between different clones were responsible for the spread of K. pneumoniae in the NICU.
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