Pone, Emanuela
(2017)
Stemness and Immunological Features of Thyroid Cancer Cells.
[Tesi di dottorato]
| Tipologia del documento: |
Tesi di dottorato
|
| Lingua: |
English |
| Titolo: |
Stemness and Immunological Features of Thyroid Cancer Cells |
| Autori: |
| Autore | Email |
|---|
| Pone, Emanuela | emanuelapone83@gmail.com |
|
| Data: |
11 Dicembre 2017 |
| Numero di pagine: |
52 |
| Istituzione: |
Università degli Studi di Napoli Federico II |
| Dipartimento: |
dep14 |
| Dottorato: |
phd054 |
| Ciclo di dottorato: |
30 |
| Coordinatore del Corso di dottorato: |
| nome | email |
|---|
| Avvedimento, Vittorio Enrico | vittorioenrico.avvedimento@unina.it |
|
| Tutor: |
| nome | email |
|---|
| Melillo, Rosa Marina | [non definito] |
|
| Data: |
11 Dicembre 2017 |
| Numero di pagine: |
52 |
| Parole chiave: |
thyroid cancer, immune escape, stemness |
| Settori scientifico-disciplinari del MIUR: |
Area 06 - Scienze mediche > MED/04 - Patologia generale |
[error in script]
[error in script]
| Depositato il: |
27 Dic 2017 23:51 |
| Ultima modifica: |
19 Mar 2019 10:13 |
| URI: |
http://www.fedoa.unina.it/id/eprint/12242 |
Abstract
Cancer stem cells (CSCs) can initiate and maintain tumours and may drive metastasis, recurrence and resistance to anti-neoplastic therapies. Recent evidence suggests that CSCs possess immunomodulatory capabilities that may enable them to evade host anti-cancer immunity to promote tumorigenicity. CSC immunological functions include evasion from immune clearance, induction of clonal anergy or deletion, and activation of regulatory immune cells. We recently identified Interleukin-8 as a crucial factor that sustains the stemness features of thyroid cancer (TC) cells through the induction, among the others, of OCT4 and SOX2 transcription factors. We generated OCT4/SOX2 overexpressing TC cells and analysed their stemness and immunomodulatory properties. We confirmed that TC cell lines overexpressing OCT4/SOX2 showed, compared to control cells, an increase in stemness features. Accordingly, OCT4/SOX2 TC cells displayed increased tumour incidence and growth when injected at limiting number in mice compared to parental cells. Negative regulators of the immune system, including Programmed cell Death-Ligands 1 and 2 (PD-L1/PD-L2) and the enzyme Indoleamine 2,3-dioxygenase (IDO) are often “hijacked” by tumours to restrain the ability of the immune system to mount an effective anti-tumor response. Here, we show that OCT4/SOX2 expressing TC cells display increased PD1, PD-L1/2 and IDO expression both at mRNA and protein levels with respect to parental cells. Consistently, TC cells overexpressing OCT4 and SOX2, when co-cultured with lymphoid cells, caused a reduction of lymphocyte vitality compared to control cells. Thus, OCT4 and SOX2 are not only key regulators of stemness features but also of immunomodulatory properties of TC cells.
Downloads per month over past year
Actions (login required)
 |
Modifica documento |
