Lettieri, Gennaro (2023) Molecular biomarkers of environmental pollution linked to the reproduction of animal species. [Tesi di dottorato]
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| Tipologia del documento: | Tesi di dottorato |
|---|---|
| Lingua: | English |
| Titolo: | Molecular biomarkers of environmental pollution linked to the reproduction of animal species |
| Autori: | Autore Email Lettieri, Gennaro gennaro.lettieri@unina.it |
| Data: | 4 Marzo 2023 |
| Numero di pagine: | 120 |
| Istituzione: | Università degli Studi di Napoli Federico II |
| Dipartimento: | Biologia |
| Dottorato: | Biologia |
| Ciclo di dottorato: | 35 |
| Coordinatore del Corso di dottorato: | nome email Esposito, Sergio sergio.esposito@unina.it |
| Tutor: | nome email Piscopo, Marina [non definito] |
| Data: | 4 Marzo 2023 |
| Numero di pagine: | 120 |
| Parole chiave: | Mytilus galloprovincialis; sperm nuclear basic proteins; mercury; heavy metals; human sperm; spermatozoa |
| Settori scientifico-disciplinari del MIUR: | Area 05 - Scienze biologiche > BIO/11 - Biologia molecolare |
| Depositato il: | 20 Mar 2023 09:21 |
| Ultima modifica: | 10 Apr 2025 14:20 |
| URI: | http://www.fedoa.unina.it/id/eprint/15209 |
Abstract
The aim of this thesis was to study the effects at the molecular level of certain potentially toxic elements (PTEs), on reproductive health in two model systems, the marine organism, Mytilus galloprovincialis, and human spermatozoa, with the objective of finding new molecular biomarkers of environmental pollution related to production. The analyses conducted on Mytilus galloprovincialis, were performed by tank exposures with artificial seawater for 24 h at 18 °C, of PTE at concentrations similar to those found in the Mediterranean Sea. Mussels exposed and not exposed to PTEs were then used for subsequent analysis, both at a protein and gene level, within the reproductive sphere. Specifically, the impact of some PTEs on Protamine- Like (PL), and in particular on their electrophoretic pattern on both urea acetic acid polyacrylamide gels in (AU-PAGE) and SDS-PAGE, was evaluated. In addition, their DNA-binding ability was evaluated by EMSA assays and their ability to protect DNA under pro-oxidation conditions. Fluorescence analyses with an ANS (8-anilinonaphthalene-1-sulphonic acid) probe and light scattering studies with CG-MALS (Composition Gradient- Multiple angle light scattering) were then conducted to assess how some of these PTEs are able to alter the conformation of PLs and their interaction with DNA or to form PL aggregates. These experimental approaches, together with others, have also been used to investigate human reproductive health by assessing possible differences, particularly of PRM properties, in individuals residing in low and high environmental impact areas in various parts of Italy. Again, the status of PRMs was evaluated with particular attention to the protamine-histone ratio, their ability to bind DNA, their ability to protect DNA under pro-oxidative conditions or to be involved in oxidative DNA damage. Specifically, the areas examined were: Terra dei Fuochi (Land of Fires, Campania region) and Valle del Sacco (Valley of Sacco rivers, Lazio region), for high environmental impact; Valle del Sele (Campania region) for low environmental impact.
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