Pota, Giulio (2024) Enzyme immobilization: design of nanoscaled supported biocatalysts and miniaturized enzyme reactors for biofuel production and green organic synthesis. [Tesi di dottorato]
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| Tipologia del documento: | Tesi di dottorato |
|---|---|
| Lingua: | English |
| Titolo: | Enzyme immobilization: design of nanoscaled supported biocatalysts and miniaturized enzyme reactors for biofuel production and green organic synthesis |
| Autori: | Autore Email Pota, Giulio giulio.pota@unina.it |
| Data: | 10 Marzo 2024 |
| Numero di pagine: | 440 |
| Istituzione: | Università degli Studi di Napoli Federico II |
| Dipartimento: | Ingegneria Chimica, dei Materiali e della Produzione Industriale |
| Dottorato: | Ingegneria dei prodotti e dei processi industriali |
| Ciclo di dottorato: | 36 |
| Coordinatore del Corso di dottorato: | nome email D'Anna, Andrea didatticadottorato.dicmapi@unina.it |
| Tutor: | nome email Costantini, Aniello [non definito] Vitiello, Giuseppe [non definito] Califano, Valeria [non definito] |
| Data: | 10 Marzo 2024 |
| Numero di pagine: | 440 |
| Parole chiave: | Enzyme immobilization; mesoporous silica nanoparticles; biofuels; organic synthesis; continuous flow biocatalysis |
| Settori scientifico-disciplinari del MIUR: | Area 03 - Scienze chimiche > CHIM/07 - Fondamenti chimici delle tecnologie |
| Depositato il: | 20 Mar 2024 07:23 |
| Ultima modifica: | 18 Mar 2026 10:43 |
| URI: | http://www.fedoa.unina.it/id/eprint/15497 |
Abstract
This PhD thesis presents successful methodologies for designing nanoscaled biocatalysts and implementing continuous-flow enzymatic processes for green chemistry applications. Wrinkled silica nanoparticles (WSNs) are identified as ideal supports for protein immobilization, particularly for cellulases and lipases, due to their unique morphological and chemical properties. The ability to adjust pore size and interwrinkle distance facilitates high enzyme loads, enhancing glucose production from cellulose hydrolysis. Physical immobilization techniques, without chemical linkers, yield reusable and thermally stable biocatalysts. The thesis also explores miniaturized enzyme reactors, exemplified by a telescoped multi-enzyme hydrolysis process, demonstrating performance and reusability. Overall, the findings underscore the importance of enzyme immobilization in advancing biocatalytic processes towards industrial scalability and sustainability.
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