Di Tolla, Michele Francesco (2024) Comparative Cytokine Dynamics: Unraveling the role of Lifestyle and Dietary Factors in shaping Low Grade Chronic Inflammation across Healthy and Neoplastic Profiles. [Tesi di dottorato]

Anteprima

Testo Di Tolla_Michele_36.pdf Download (2MB) | Anteprima

Abstract

Low Grade Chronic Inflammation (LGCI) is a common feature of noncommunicable diseases. Cytokines play a crucial role in LGCI. This study aimed to assess how LGCI risk factors [e.g., age, body mass index (BMI), smoke, physical activity, and diet] may impact on specific cytokine levels in both healthy and neoplastic populations. In total, 150 healthy volunteers and 132 neoplastic patients were recruited and subjected to questionnaires about the last 7-days lifestyle, including smoking habit, physical activity, and food frequency. A panel of circulating cytokines, chemokines, growth factors, metabolic markers and adipokines was analyzed by multiplex ELISA. In healthy patients, BMI showed the heaviest impact on the correlation between LGCI-related risk factors and cytokines and was significantly associated with CRP levels. BMI, along with aging, smoking, physical activity, and eating habits were associated with an inflammatory pattern, characterized by a cluster that included IL-1β, IL-6, IL-8 and TNF-α. These markers retained their strong clustering feature in the neoplastic population, with the addition of other inflammatory markers, such as MIP-1α, MIP-1β, IL-4, and GM-CSF. In conclusion, BMI, age, smoke, physical activity, and dietary habits are associated with specific cytokines that may represent potential markers for LGCI, and a first proof of this biomarker validation is given by the reappearance of this cluster in a neoplastic population.

Downloads

Actions (login required)

Modifica documento