Limatola, Antonio (2015) NMR spectroscopy: a useful tool for drugs development and monitoring biological reactions. [Tesi di dottorato]
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| Tipologia del documento: | Tesi di dottorato |
|---|---|
| Lingua: | English |
| Titolo: | NMR spectroscopy: a useful tool for drugs development and monitoring biological reactions |
| Autori: | Autore Email Limatola, Antonio antonio.limatola@unina.it |
| Data: | 31 Marzo 2015 |
| Numero di pagine: | 137 |
| Istituzione: | Università degli Studi di Napoli Federico II |
| Dipartimento: | Farmacia |
| Scuola di dottorato: | Scienze farmaceutiche |
| Dottorato: | Scienza del farmaco |
| Ciclo di dottorato: | 27 |
| Coordinatore del Corso di dottorato: | nome email D'Auria, Maria Valeria madauria@unina.it |
| Tutor: | nome email Carotenuto, Alfonso [non definito] |
| Data: | 31 Marzo 2015 |
| Numero di pagine: | 137 |
| Parole chiave: | NMR spectroscopy; conformational analysis; drug development; in-cell NMR |
| Settori scientifico-disciplinari del MIUR: | Area 05 - Scienze biologiche > BIO/10 - Biochimica Area 05 - Scienze biologiche > BIO/11 - Biologia molecolare Area 03 - Scienze chimiche > CHIM/02 - Chimica fisica Area 03 - Scienze chimiche > CHIM/08 - Chimica farmaceutica |
| Depositato il: | 10 Apr 2015 11:52 |
| Ultima modifica: | 28 Apr 2018 01:00 |
| URI: | http://www.fedoa.unina.it/id/eprint/10433 |
| DOI: | 10.6093/UNINA/FEDOA/10433 |
Abstract
Background of my doctoral PhD program is primarily based on the use of nuclear magnetic resonance spectroscopy (NMR) as a versatile tool that can be applied for several biological purposes. During the early years of my PhD, I mainly used proton detection nuclear magnetic resonance techniques (1H-NMR) for the identification and characterization of molecules that can act as potential drugs. Since, GPCRs are targeted by approximately 45% to 50% of medicinal drugs, I focused my attention on the development and conformational analysis of peptides modulating a few of these receptors bringing to different therapeutic effects. During the last period of my PhD, I used NMR spectroscopy techniques based on 15N and/or 13C detection. Techniques suitable to study much larger systems as whole proteins. I applied in-cell, in extracts and in vitro NMR spectroscopy techniques to study and monitor in a qualitative and quantitative manner biological reactions that can occur on a protein into a biological environment. In particular, I studied the intracellular fate of oxidized α‑synucein molecules.
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