Limatola, Antonio (2015) NMR spectroscopy: a useful tool for drugs development and monitoring biological reactions. [Tesi di dottorato]
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Testo
Limatola_Antonio_27.pdf Download (6MB) | Anteprima |
| Tipologia del documento: | Tesi di dottorato | ||||
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| Lingua: | English | ||||
| Titolo: | NMR spectroscopy: a useful tool for drugs development and monitoring biological reactions | ||||
| Autori: |
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| Data: | 31 Marzo 2015 | ||||
| Numero di pagine: | 137 | ||||
| Istituzione: | Università degli Studi di Napoli Federico II | ||||
| Dipartimento: | Farmacia | ||||
| Scuola di dottorato: | Scienze farmaceutiche | ||||
| Dottorato: | Scienza del farmaco | ||||
| Ciclo di dottorato: | 27 | ||||
| Coordinatore del Corso di dottorato: |
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| Tutor: |
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| Data: | 31 Marzo 2015 | ||||
| Numero di pagine: | 137 | ||||
| Parole chiave: | NMR spectroscopy; conformational analysis; drug development; in-cell NMR | ||||
| Settori scientifico-disciplinari del MIUR: | Area 05 - Scienze biologiche > BIO/10 - Biochimica Area 05 - Scienze biologiche > BIO/11 - Biologia molecolare Area 03 - Scienze chimiche > CHIM/02 - Chimica fisica Area 03 - Scienze chimiche > CHIM/08 - Chimica farmaceutica |
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| Depositato il: | 10 Apr 2015 11:52 | ||||
| Ultima modifica: | 28 Apr 2018 01:00 | ||||
| URI: | http://www.fedoa.unina.it/id/eprint/10433 | ||||
| DOI: | 10.6093/UNINA/FEDOA/10433 |
Abstract
Background of my doctoral PhD program is primarily based on the use of nuclear magnetic resonance spectroscopy (NMR) as a versatile tool that can be applied for several biological purposes. During the early years of my PhD, I mainly used proton detection nuclear magnetic resonance techniques (1H-NMR) for the identification and characterization of molecules that can act as potential drugs. Since, GPCRs are targeted by approximately 45% to 50% of medicinal drugs, I focused my attention on the development and conformational analysis of peptides modulating a few of these receptors bringing to different therapeutic effects. During the last period of my PhD, I used NMR spectroscopy techniques based on 15N and/or 13C detection. Techniques suitable to study much larger systems as whole proteins. I applied in-cell, in extracts and in vitro NMR spectroscopy techniques to study and monitor in a qualitative and quantitative manner biological reactions that can occur on a protein into a biological environment. In particular, I studied the intracellular fate of oxidized α‑synucein molecules.
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